LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Abstract Background LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet be fully investigated. Our study focused chiefly on interaction and role of HOXA-AS2/miR-885-5p/RBBP4 axis development Methods qRT-PCR analysis was performed detect expression lncRNA, miRNA mRNA glioblastoma tissues cells. Dual-luciferase assay, RIP assay RNA pull-down were later carried out reveal interactions among HOXA-AS2, miR-885-5p RBBP4. After that, CCK-8 BrdU nude mice xenografting western blot flow cytometry analyze effect samples. Results RBBP4 overexpressed Experimental results showed that contributed tumorigenesis observed downregulated Findings also indicated could negatively regulate miR-885-5p, thereby enhancing expression. Conclusion Overall, promoted by targeting regulating induce